Saccharomyces cerevisiae’de Krom (K2Cr2O7) ile Oluşturulan Oksidatif Hasara Karşı Goji Berry (Lycium barbarum L.)’nin Koruyucu Etkileri
نویسندگان
چکیده
Goji berry (Lycium barbarum L.) antioksidan özellikleri sayesinde son zamanlarda kullanılan fonksiyonel gıdalar arasında yer almaktadır. Anti-oksidatif, kardiyo-protektif, nöro-protektif, anti-diyabet, anti-kanser ve anti-tümör özelliklerinin yanı sıra daha birçok hastalığın tedavisinde koruyucu rol oynadığı bilinmektedir. Bu çalışmada 4 grup oluşturulmuştur. Gruplar; (1) Kontrol grubu; (2) Grubu (%10); (3) Krom (K2Cr2O7) (10 mM); (4) (% 10) + mM) Grubu. Saccharomyces cerevisiae kültürleri 1h, 3h, 5h 24h boyunca 30 °C'de geliştirilmiştir. Malondialdehit (MDA) düzeyleri, katalaz aktivite tayini (CAT), glutatyon (GSH) seviyeleri hücre gelişimi spektrofotometre ile ölçülmüştür. SDS-PAGE elektroforez analizi total protein değişiklikleri tespit edilmiş bradford metodu hesaplanmıştır. Çalışma sonuçlarımıza göre; grubu kıyaslandığında, grubunda (1h, 24h, sentezi (24h), GSH (24h) aktiviteleri artarken, MDA düzeyi azalış göstermiştir. Sonuç olarak, yaprağı’nın S. kültüründe kaynaklı oksidatif hasarı azaltarak, sentezini olumlu yönde arttırdığı belirlenmiştir. yaprağı tedavisinin büyümesinde teşvik edici bir role sahip olduğu edilmiştir.
منابع مشابه
Comparative studies on phenolic profiles, antioxidant capacities and carotenoid contents of red goji berry (Lycium barbarum) and black goji berry (Lycium ruthenicum)
BACKGROUND The study on phytochemical difference between red and black goji berry is limited. METHODS Antioxidant activities and phenolic profiles in terms of total phenol content, total flavonoid contents, condensed tannin content, monomeric anthocyanin content, and total carotenoid content of red goji berry (Lycium barbarum) and black goji berry (L. ruthenicum) were compared using colorimet...
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Lycium barbarum (goji berry) has long been used as a food and traditional herbal medicine. This study aimed to investigate the beneficial effect of the goji berry on dry eye disease in rats. Male Sprague‑Dawley rats with induced dry eye disease were randomly assigned to four groups: Vehicle (control), low‑dose goji berry extract [GBE; 250 mg/kg/body weight (bw)], median‑dose GBE (350 mg/kg/bw),...
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Goji berry (wolfberry), a member of the Solanacea family, has been recently introduced in Western countries and its consumption has increased rapidly. The objectives of the study were to describe the cases of 2 patients who experienced allergic symptoms after Goji berry consumption, to identify the protein profile of the extract, to analyze the allergenic profile of individuals, and to determin...
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Although Lycium barbarum (goji) and active compounds, Lycium barbarum polysaccharides (LBP), have a high in vitro antioxidant score as determined by simple chemical reaction methods, their in vivo antioxidant effects in humans have not been extensively examined. After our earlier report that an LBP-standardized Lycium barbarum preparation (GoChi) helps prevent oxidant stress-related conditions ...
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ژورنال
عنوان ژورنال: Bitlis Eren üniversitesi fen bilimleri dergisi
سال: 2021
ISSN: ['2147-3188', '2147-3129']
DOI: https://doi.org/10.17798/bitlisfen.904623